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mitomycin c treated sto cell feeder layer  (ATCC)


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    Structured Review

    ATCC mitomycin c treated sto cell feeder layer
    Mitomycin C Treated Sto Cell Feeder Layer, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1749 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mitomycin+c+treated+sto+cell+feeder+layer/A-10/pm26053518-53-35-41
    Average 97 stars, based on 1749 article reviews
    mitomycin c treated sto cell feeder layer - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Modification:

    Article Title: Stem Cell–Derived Bioactive Materials Accelerate Development of Porcine In Vitro –Fertilized Embryos
    Article Snippet: .. Preparation of hESC-BM JNU-hES-01 cells (15–20 passages), a hESC line established at Jeju National University using a method modified from Kim et al. ( 2011 ), were maintained in an undifferentiated state by culturing the cells on a 10 μg/mL mitomycin C–treated STO cell feeder layer [American Type Culture Collection (ATCC), USA) in hESC culture medium [80% DMEM, Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut Serum Replacement (Gibco), 0.1 mM β-mercaptoethanol, 1% nonessential amino acids, and 4 ng/mL hbFGF (KOMA Biotech, Inc.)]. ..

    Article Title: Stem Cell-Derived Bioactive Materials Accelerate Development of Porcine In Vitro-Fertilized Embryos.
    Article Snippet: .. JNU-hES-01 cells (15–20 passages), a hESC line established at Jeju National University using a method modified from Kim et al. (2011), were maintained in an undifferentiated state by culturing the cells on a 10 lg/mL mitomycin C–treated STO cell feeder layer [American Type Culture Collection (ATCC), USA) in hESC culture medium [80% DMEM, Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut Serum Replacement (Gibco), 0.1 mM b-mercaptoethanol, 1% nonessential amino acids, and 4 ng/mL hbFGF (KOMA Biotech, Inc.)]. ..

    Article Title: Stem Cell–Derived Bioactive Materials Accelerate Development of Porcine In Vitro –Fertilized Embryos
    Article Snippet: .. JNU-hES-01 cells (15–20 passages), a hESC line established at Jeju National University using a method modified from Kim et al. ( 2011 ), were maintained in an undifferentiated state by culturing the cells on a 10 μg/mL mitomycin C–treated STO cell feeder layer [American Type Culture Collection (ATCC), USA) in hESC culture medium [80% DMEM, Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut Serum Replacement (Gibco), 0.1 mM β-mercaptoethanol, 1% nonessential amino acids, and 4 ng/mL hbFGF (KOMA Biotech, Inc.)]. ..

    Article Title: The use of embryonic stem cell derived bioactive material as a new protein supplement for the in vitro culture of bovine embryos.
    Article Snippet: Groups of ten COCs were cultured in 50-μl droplets of maturation medium (TCM-199, Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Gibco), 0.2 mM sodium pyruvate, 1 μg/ml FSH (Folltropin-V; Bioniche Animal Health, Belleville, ON, Canada), 10 μg/ml LH (Leutropin; Bioniche Animal Health), 1 μg/ml estradiol-17β and 25 μg/ml gentamycin. .. Droplets were covered with mineral oil and incubated in the presence of 5% CO2, 5% O2 and 90% N2 at 38.8 C. Preparation of human embryonic stem cell derived bioactive material (hES-BM) JNU-hES-01 cells (15–20 passages), a human embryonic stem (hES) cell line established at Jeju National University using a modified Park’s method [11], were maintained in an undifferentiated state by culturing the cells on a 10 μg/ml mitomycin C-treated STO cell feeder layer (ATCC, USA) in hES cell culture medium [80% Dulbecco’s Modified Eagle’s Medium (DMEM) Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut serum replacement (Gibco), 0.1 mM β-mercaptoethanol, 1% nonessential amino acids and 4 ng/ml human basic fibroblast growth factor (hbFGF, KOMA Biotech, Inc)]. ..

    Knock-Out:

    Article Title: Stem Cell–Derived Bioactive Materials Accelerate Development of Porcine In Vitro –Fertilized Embryos
    Article Snippet: .. Preparation of hESC-BM JNU-hES-01 cells (15–20 passages), a hESC line established at Jeju National University using a method modified from Kim et al. ( 2011 ), were maintained in an undifferentiated state by culturing the cells on a 10 μg/mL mitomycin C–treated STO cell feeder layer [American Type Culture Collection (ATCC), USA) in hESC culture medium [80% DMEM, Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut Serum Replacement (Gibco), 0.1 mM β-mercaptoethanol, 1% nonessential amino acids, and 4 ng/mL hbFGF (KOMA Biotech, Inc.)]. ..

    Article Title: Stem Cell-Derived Bioactive Materials Accelerate Development of Porcine In Vitro-Fertilized Embryos.
    Article Snippet: .. JNU-hES-01 cells (15–20 passages), a hESC line established at Jeju National University using a method modified from Kim et al. (2011), were maintained in an undifferentiated state by culturing the cells on a 10 lg/mL mitomycin C–treated STO cell feeder layer [American Type Culture Collection (ATCC), USA) in hESC culture medium [80% DMEM, Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut Serum Replacement (Gibco), 0.1 mM b-mercaptoethanol, 1% nonessential amino acids, and 4 ng/mL hbFGF (KOMA Biotech, Inc.)]. ..

    Article Title: Stem Cell–Derived Bioactive Materials Accelerate Development of Porcine In Vitro –Fertilized Embryos
    Article Snippet: .. JNU-hES-01 cells (15–20 passages), a hESC line established at Jeju National University using a method modified from Kim et al. ( 2011 ), were maintained in an undifferentiated state by culturing the cells on a 10 μg/mL mitomycin C–treated STO cell feeder layer [American Type Culture Collection (ATCC), USA) in hESC culture medium [80% DMEM, Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut Serum Replacement (Gibco), 0.1 mM β-mercaptoethanol, 1% nonessential amino acids, and 4 ng/mL hbFGF (KOMA Biotech, Inc.)]. ..

    Article Title: The use of embryonic stem cell derived bioactive material as a new protein supplement for the in vitro culture of bovine embryos.
    Article Snippet: Groups of ten COCs were cultured in 50-μl droplets of maturation medium (TCM-199, Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Gibco), 0.2 mM sodium pyruvate, 1 μg/ml FSH (Folltropin-V; Bioniche Animal Health, Belleville, ON, Canada), 10 μg/ml LH (Leutropin; Bioniche Animal Health), 1 μg/ml estradiol-17β and 25 μg/ml gentamycin. .. Droplets were covered with mineral oil and incubated in the presence of 5% CO2, 5% O2 and 90% N2 at 38.8 C. Preparation of human embryonic stem cell derived bioactive material (hES-BM) JNU-hES-01 cells (15–20 passages), a human embryonic stem (hES) cell line established at Jeju National University using a modified Park’s method [11], were maintained in an undifferentiated state by culturing the cells on a 10 μg/ml mitomycin C-treated STO cell feeder layer (ATCC, USA) in hES cell culture medium [80% Dulbecco’s Modified Eagle’s Medium (DMEM) Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut serum replacement (Gibco), 0.1 mM β-mercaptoethanol, 1% nonessential amino acids and 4 ng/ml human basic fibroblast growth factor (hbFGF, KOMA Biotech, Inc)]. ..

    Incubation:

    Article Title: The use of embryonic stem cell derived bioactive material as a new protein supplement for the in vitro culture of bovine embryos.
    Article Snippet: Groups of ten COCs were cultured in 50-μl droplets of maturation medium (TCM-199, Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Gibco), 0.2 mM sodium pyruvate, 1 μg/ml FSH (Folltropin-V; Bioniche Animal Health, Belleville, ON, Canada), 10 μg/ml LH (Leutropin; Bioniche Animal Health), 1 μg/ml estradiol-17β and 25 μg/ml gentamycin. .. Droplets were covered with mineral oil and incubated in the presence of 5% CO2, 5% O2 and 90% N2 at 38.8 C. Preparation of human embryonic stem cell derived bioactive material (hES-BM) JNU-hES-01 cells (15–20 passages), a human embryonic stem (hES) cell line established at Jeju National University using a modified Park’s method [11], were maintained in an undifferentiated state by culturing the cells on a 10 μg/ml mitomycin C-treated STO cell feeder layer (ATCC, USA) in hES cell culture medium [80% Dulbecco’s Modified Eagle’s Medium (DMEM) Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut serum replacement (Gibco), 0.1 mM β-mercaptoethanol, 1% nonessential amino acids and 4 ng/ml human basic fibroblast growth factor (hbFGF, KOMA Biotech, Inc)]. ..

    Derivative Assay:

    Article Title: The use of embryonic stem cell derived bioactive material as a new protein supplement for the in vitro culture of bovine embryos.
    Article Snippet: Groups of ten COCs were cultured in 50-μl droplets of maturation medium (TCM-199, Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Gibco), 0.2 mM sodium pyruvate, 1 μg/ml FSH (Folltropin-V; Bioniche Animal Health, Belleville, ON, Canada), 10 μg/ml LH (Leutropin; Bioniche Animal Health), 1 μg/ml estradiol-17β and 25 μg/ml gentamycin. .. Droplets were covered with mineral oil and incubated in the presence of 5% CO2, 5% O2 and 90% N2 at 38.8 C. Preparation of human embryonic stem cell derived bioactive material (hES-BM) JNU-hES-01 cells (15–20 passages), a human embryonic stem (hES) cell line established at Jeju National University using a modified Park’s method [11], were maintained in an undifferentiated state by culturing the cells on a 10 μg/ml mitomycin C-treated STO cell feeder layer (ATCC, USA) in hES cell culture medium [80% Dulbecco’s Modified Eagle’s Medium (DMEM) Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut serum replacement (Gibco), 0.1 mM β-mercaptoethanol, 1% nonessential amino acids and 4 ng/ml human basic fibroblast growth factor (hbFGF, KOMA Biotech, Inc)]. ..

    Cell Culture:

    Article Title: The use of embryonic stem cell derived bioactive material as a new protein supplement for the in vitro culture of bovine embryos.
    Article Snippet: Groups of ten COCs were cultured in 50-μl droplets of maturation medium (TCM-199, Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Gibco), 0.2 mM sodium pyruvate, 1 μg/ml FSH (Folltropin-V; Bioniche Animal Health, Belleville, ON, Canada), 10 μg/ml LH (Leutropin; Bioniche Animal Health), 1 μg/ml estradiol-17β and 25 μg/ml gentamycin. .. Droplets were covered with mineral oil and incubated in the presence of 5% CO2, 5% O2 and 90% N2 at 38.8 C. Preparation of human embryonic stem cell derived bioactive material (hES-BM) JNU-hES-01 cells (15–20 passages), a human embryonic stem (hES) cell line established at Jeju National University using a modified Park’s method [11], were maintained in an undifferentiated state by culturing the cells on a 10 μg/ml mitomycin C-treated STO cell feeder layer (ATCC, USA) in hES cell culture medium [80% Dulbecco’s Modified Eagle’s Medium (DMEM) Nutrient Mixture F-12 (1:1, Gibco), 20% KnockOut serum replacement (Gibco), 0.1 mM β-mercaptoethanol, 1% nonessential amino acids and 4 ng/ml human basic fibroblast growth factor (hbFGF, KOMA Biotech, Inc)]. ..



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